Reduction of Accumulated Reactive Oxygen Species Can Be Achieved By Bathing Standard Lipoaspirate in Oxygenated Micro/Nanobubbles
Derek Banyard, MD, MBA, Ryan S. Chiang, n/a, Nikos Sarantopoulos, n/a, Anna A. Borovikova, MD, Michael J. Klopfer, PhD, Garrett A. Wirth, MD, MS, Keyianoosh Z. Paydar, MD, Mark Bachman, PhD, Gregory R.D. Evans, MD, Alan D. Widgerow, MBBCh, MMed.
University of California, Irvine, Orange, CA, USA.
PURPOSE: Micro/Nanobubbles (MNBs) are gaining attention in the field of medicine due to their stability in solution and potential as a therapeutic delivery system. Here, we tested whether oxygenated MNBs could be used to reduce the effects of hypoxia on standard liposuction aspirate.
METHODS: Lipoaspirate from a routine liposuction case was maintained at room temperature for 24 hours. Oxygenated MNBs were infused into phosphate buffered saline (PBS) to a concentration >20 mg/L using our custom MNB generator. The lipoaspirate was bathed in either PBS or PBS + MNBs for 15, 30 or 60 minutes on a rocker. At each time point, samples were snap frozen in liquid nitrogen, cut to a thickness of 25 μm and stained with dihydroethidium (DHE) and 4',6-diamidino-2-phenylindole (DAPI). Images were obtained using a fluorescence microscope. Positive nuclear staining (DAPI) versus reactive oxygen species (ROS) staining (DHE) were quantified and represented as a ratio (DAPI/DHE).
RESULTS: Standard lipoaspirate that was bathed in oxygenated MNBs expressed a significant reduction in accumulated ROS at 15 and 30 minutes when compared to the control group (1.01 ± 0.04 vs 0.85 ± 0.13, p = 0.05 and 1.27 ± 0.22 vs. 1.10 ± 0.14, p = 0.05 respectively). There was no comparable reduction in ROS for either group at 1 hour.
CONCLUSION: Buffered saline oxygenated with micro/nanobubbles can be used as a bathing solution for lipoaspirate to mitigate the deleterious effects of hypoxia including the generation of ROS. Further studies are needed to determine the clinical significance of these findings.
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