Adipose-derived Stem Cells Promote Wound Healing of Irradiated Abdominal Muscle in a Rat Model
Youbai Chen, MD, Qixu Zhang, MD,PhD, Yewen Wu, MS, Cynthia D. Branch-Brooks, BS, Charles E Butler, MD.
University of Texas, MD Anderson Cancer Center, Houston, TX, USA.
Purpose:Owing to their adult stem cell characteristics and easy isolation with conventional liposuction procedures, adipose-derived stem cells (ASCs)-based treatments have been expanded substantially in recent years. However, the therapeutic effect of ASCs treatment on irradiated muscle tissue is still poor understood. This study aimed to determine whether ASCs could facilitate wound healing of irradiated abdominal muscle in a rat model.
Methods: Aged Fischer rats with varies dose irradiation on abdomen were underwent laparotomy based on our established protocol. Rats were divided randomly into 6 groups according to the radiological dose and with or without intramuscular injections of ASCs isolated from healthy rats (Table1). 14 days postoperatively, muscle samples were harvested and divided into 3 pieces for mechanical tests, histological and immunohistochemical staining, and protein array analysis.
Results: Mechanical tests showed that mechanical properties of abdominal muscle were impaired by radiation and enhanced by ASCs treatment. Elastic modulus (EM) (0.88±0.35MPa) and ultimate tensile strength (UTS) (0.35±0.10KPa) of Group 4 were significantly strong than Group 3(p<0.05) (Figure1). H&E staining indicated that ASCs mitigated inflammatory cell infiltration by migration and localization to the wound interphase. A higher expression of CD31 in endothelial cells and increased capillary density were observed in ASCs treated rats (p<0.05) which suggested that ASCs stimulated new blood vessel formation. Masson Trichrome and MyoD1 staining showed that ASCs treatment improved collagen deposition and induced muscle regeneration (p<0.05).
Conclusions: ASCs accelerate wound healing of irradiated abdominal muscle via their capability to modulate inflammation, induce angiogenesis and activate myogenesis.
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